摘要拟南芥种子萌发过程中茉莉酸对脱落酸信

北京有哪些治疗白癜风的医院 http://m.39.net/disease/yldt/bjzkbdfyy/

勤读立耕立己达人

Mar18

,THU

第期

第一作者：JinjingPan

通讯单位：云南大学

通讯作者：DiqiuYu*(ydq

ynu.edu.cn)

出版期刊：ThePlantCell()

MolecularMechanismUnderlyingtheSynergeticEffectofJasmonateonAbscisicAcidSignalingduringSeedGerminationinArabidopsis

拟南芥种子萌发过程中茉莉酸对脱落酸信号的协同作用的分子机制

Abstract

Abscisicacid(ABA)isknowntosuppressseedgerminationandpost-germinativegrowthofArabidopsis(Arabidopsisthaliana),andjasmonate(JA)enhancesABAfunction.However,themolecularmechanismunderlyingthecrosstalkbetweentheABAandJAsignalingpathwaysremainslargelyelusive.

Here,weshowthatexogenouscoronatine,aJAanalogstructurallysimilartotheactiveconjugatejasmonate-isoleucine,significantlyenhancesthedelayedseedgerminationresponsetoABA.DisruptionoftheJAreceptorCORONATINEINSENSITIVE1oraccumulationoftheJAsignalingrepressorJASMONATEZIM-DOMAIN(JAZ)reducedABAsignaling,whilejazmutantsenhancedABAresponses.

MechanisticinvestigationsrevealedthatseveralJAZrepressorsofJAsignalingphysicallyinteractwithABSCISICACIDINSENSITIVE3(ABI3),acriticaltranscriptionfactorthatpositivelymodulatesABAsignaling,andthatJAZproteinsrepressthetranscriptionofABI3andABI5.

FurthergeneticanalysesshowedthatJAactivatesABAsignalingandrequiresfunctionalABI3andABI5.OverexpressionofABI3andABI5simultaneouslysuppressedtheABA-insensitivephenotypesofthecoi1-2mutantandJAZ-accumulating(JAZ-ΔJas)plants.

Together,ourresultsrevealapreviouslyuncharacterizedsignalingmoduleinwhichJAZrepressorsoftheJApathwayregulatetheABA-responsiveABI3andABI5transcriptionfactorstointegrateJAandABAsignalsduringseedgerminationandpost-germinativegrowth.

摘要

脱落酸(ABA)可抑制拟南芥种子萌发和萌发后的生长，而茉莉酸(JA)能增强ABA的功能。然而，ABA和JA信号途径之间的潜在串扰分子机制依然不清楚。本研究中，作者发现外源冠菌素(一种与茉莉酸-异亮氨酸活性共轭的JA结构类似物)显著增强了种子对ABA的延迟萌发响应。对JA受体CORONATINEINSENSITIVE1(COI1)的干扰或JA信号抑制子JAZ可降低ABA信号，而jaz突变体则可增强对ABA的响应。研究表明，几个JA信号中的JAZ抑制子可与ABSCISICACIDINSENSITIVE3(ABI3)发生互作，ABI3是正调控ABA信号的关键调控因子，而JAZ蛋白可抑制ABI3和ABI5的转录。进一步的遗传分析表明，JA需要ABI3和ABI5功能来激活ABA信号。ABI3和ABI5的过表达都能挽救coi1-2突变体和JAZ积累(JAZ-ΔJas)植株的ABA不敏感表型。综上，本研究结果揭示了一个以前未被表征的信号转导模型，JA途径中的JAZ抑制子通过调节ABA响应的ABI3和ABI5转录因子，从而整合了种子萌发和萌发后生长的JA和ABA信号。

END

论文链接：
转载请注明原文网址：http://www.tumushukezx.com/tmskshj/11284.html